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This process is repeated, without cleaning and sanitizing for an additional 2 days. The reasoning behind this follow-up step is to show that the contamination, if spread, has been eliminated. After sanitizing is completed, additional swabs are then used to sample the initial positive site and surrounding areas again. Once the initial positive site and surroundings have been sampled, the area should be appropriately cleaned and sanitized. Next, we take swabs of the initial positive site and around the positive site in a circular pattern. When swabbing, select a representative area and sites around the initial positive site, see figure 1. Not sure how to take a swab? Check out this video. Best practices suggest using at least five swabs in varying directions away from the initial positive site for 3 consecutive days. The key to vector swabbing is to take additional swabs around the initial positive site to see if the contamination has spread. Discovering the root cause empowers you to conduct aggressive corrective actions such as reenforcing GMPs, changing traffic patterns, repairing and modifying equipment, all of which could help prevent contamination and delays in the future. With each new swab, it brings you another clue closer to your answer or root cause. Think of it like this: you are a detective trying to solve the mystery as to why you got a presumptive positive or OOS result and if the contamination has spread. It is best to immediately quarantine the area where the indicator organism was found to prevent any spread of the contamination. You can begin this type of swabbing once the presumptive positive notification is received, or after a confirmed positive result is received. Vector swabbing is an additional investigative process in your Environmental Monitoring Program (EMP). The answer: Best practices suggests vector swabbing. However, you also received an out of spec (OOS) count for an indicator organism and/or a presumptive positive notification for a pathogen. You receive the results, and you’ve gotten mostly negative or acceptable counts, which is excellent.

You have just finished your swabbing, and now you wait with anticipation for the results to come back from the lab.